Since the first description of the genus Brevibacterium in 1953 by Breed,1 it had been rarely reported as a cause of catheter related bloodstream infection (CRBSI). This microorganism was considered apothegenic until a few reports of infections in immunocompromised patients were published. Coryneform bacteria are aerobically growing Gram-positive rods with distinctive irregular morphology on staining. Taxonomically, they are a heterogenous group consisting of various genera, including the genus Corynebacterium and the genus Brevibacterium. Brevibacteria are nonmotile, nonfastidous, chemoorganothrophic, obligately aerobic, rod-shaped, halotolerant (≥6.5% NaCl), and catalase positive.2 In nature, Brevibacterium contributes notably to the aroma and color (orange pigment) of surface-ripened cheese. The organism can also be found in raw milk, human skin, and animal sources. Presently, the genus Brevibacterium consists of 45 different species, of which only ten, namely, B. linens, B. casei, B. epidermidis, B. iodinum, B. mcbrellneri, B. otitidis, B. paucivorans, B. sanguinis, B. massiliense, B. avium have been isolated from clinical samples.3B. casei is the most frequently isolated Brevibacterium species from otherwise sterile human sites.3 In English literature among the eight patients with B. Casei catheter-related bacteremia reported, three were pediatric patients.4–11 This is the first case of B. casei CRBSI in a child with acute leukemia.

A 6-year-old male child with standard-risk group B-cell acute lymphoblastic leukemia (ALL) was being treated according to BFM-2002, in the maintenance phase of a chemotherapeutic regimen that included methotrexate and 6-mercaptopurine was admitted to our hospital complaining of small grouped maculopapular lesions on an erythematous base. The papules rapidly turned into vesicles, then into pustules in the lumbar area. The patient received intravenous acyclovir for herpes zoster infection. He developed fever on the fifth day of hopitalization and his whole blood cell count revealed pancytopenia; he was then put on piperacillin-tazobactam (75mg/kg every 8h) empirically according to febrile neutropenia guidelines. Intravenous vancomycin (15mg/kg every 6h) was added because the patient had a history of coagulase-negative staphylococcus CRBSI. The complete blood cell count (CBC) at the time of fever included a white blood cell count of 1930mm3/μL (reference range, [4–10.2]×103mm3/μL), hemoglobin of 7.24g/dL (reference range, 11–16g/dL), and a platelet count of 85×103mm3/μL (reference range, [150–400]×103mm3/μL), absolute neutrophil count of 387mm3/μL (reference range, [1.5–6]×103mm3/μL). C-reactive protein was 6.1mg/dL. A complete metabolic panel turned out normal, with no evidence of liver or renal dysfunction. The patient was febrile with an ill appearance but maintained normal blood pressure and normal mental status, and there was no concern for sepsis. All three blood cultures collected following fever turned out positive with the same coryneform bacteria Brevibacterium casei. Antimicrobial susceptibility testing revealed that the organism was susceptible to vancomycin and fever resolved after 24h of antibiotic therapy and was not switched.

Two follow-up blood cultures were collected in the subsequent week of incubation. These cultures came back negative. The patient finally defervesced 10 days after starting therapy.

Positive blood cultures that were collected through the Hickmann catheter suggested that the portal of entry of the organisms was related to compromised mucosal integrity secondary to the indwelling central line.

Catheter removal was not required and bacteremia due to the same pathogen for more than six months has not recurred.

The organism was recovered in the BacT/Alert system (bioMérieux, France) from both the aerobic and anaerobic bottles of all three blood culture sets. On Gram-stained smears from the culture bottles, the organisms appeared as Gram-positive, club-shaped, slightly curved rods, and some coccal forms were also present. Colonies on sheep blood and chocolate agars were gray-white, smooth, non-hemolytic, and had a pungent cheese-like odor.

Presumptive identification of Brevibaterium spp was made and the isolate was further identified by VITEK MS (bioMérieux, France). Vitek-MS that is using Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) technology which is a new technology for species identification based on the protein composition of microbial cells. The most prominent advantages are the quick turnaround time and its low cost to access a quality database of reference spectra, including Brevibacteria.12 The isolate was tested for antibiotic sensitivity on Muller Hinton agar by Kirby Bauer disc diffusion technique using standard methods. The strain was sensitive to all antibiotics tested, i.e. penicillin, cephalothin, cefotaxime, gentamicin, erythromycin, ciprofloxacin, and vancomycin as per Clinical and Laboratory Standards Institute (CLSI) guidelines. CLSI for interpreting susceptibility results are based on the recommandations that apply to Corynebacterium species.13

The most common organisms isolated from pediatric CRBSI are coagulase-negative staphylococci (CNS) and Staphylococcus aureus. Gram-negative bacilli, Candida spp., and enterococci can be isolated, especially in neonates.14 Brevibacteria are catalase-positive, non-spore-forming, nonmotile, aerobic Gram-positive rods. They can be found in raw milk and surface-ripened cheese as well as on human skin and in animal sources. Brevibacterium spp. are vary rare pathogens. Therefore, they were not considered human pathogens until case reports have been published.4–11

The first pediatric patient reported in the literature had a diagnosis of neuroblastoma and was non-neutropenic at the time of B. casei septicemia, the catheter was removed but antibiotic therapy was not reported.6 Reinert et al.4 reported on a 25-year-old boy with a diagnosis of testicular choriocarcinoma; while he was receiving chemotherapy he developed pancytopenia and fever, and the hemoculture revealed B. casei. The reported patient was treated with piperacillin and teicoplanin for the first 10 days, and in the subsequent 10 days he was on piperacillin and tobramycin. He relapsed two weeks after therapy was stopped. Brazzola et al.7 reported on an 18-year-old girl with AIDS, who developed persisting fever and dehydratation and had a port-a-cath. Hemoculture and port-a-cath culture both revealed B. casei and the patient was treated with ciprofloxacin for 14 days and the port-a-cath was removed. She had no relapses. Janda et al.8 reported on a 34-year-old man with AIDS, who had persisting fever and pancytopenia. The culture from Hickmann catheter and hemoculture both revealed B. casei. The patient was given vancomycin for eight days and the catheter was removed. He had no relapses. Ulrich et al.9 reported on a 62-year-old man, who was treated with continuous-intravenous iloprost via non-tunneled central venous catheter (CVC) for severe pulmonary hypertension. The patient developed fever, chills, cough and dyspnea, and the culture from peripheral vein and CVC both revealed B. casei. He was treated with vancomycin for 10 days and moxifloxacin for the subsequent 21 days; the catheter was removed, no recurrence occurred. Our patient had Hickmann catheter, he was treated with vancomycin for 10 days but the catheter was not removed. He had no relapses after B. casei septicemia on the following six months.

Brevibacterium spp. was not considered human pathogens until few cases were published in the literature. All reported cases treated with combination of various antibiotics, especially glycopeptides and quinolones, and catheter removal. The recent patient was treated with vancomycin, the catheter was not removed because of ongoing chemotherapy, no recurrence occurred in the following six months. Patients with indwelling central venous catheters are at high risk of acquiring bloodstream infections. A variety of unusual pathogens may be encountered, especially in immunocompromised patients. Among these, Brevibacterium spp. are rarely found and can be confused with apathogenic corynebacteria. Physicians treating patients with cytotoxic chemotherapeutic regimens should be aware of this bacterial genus as a potential cause of invasive infection.

The authors declare no conflicts of interest.