Journal Information
Vol. 19. Issue 1.
Pages 82-84 (January - February 2015)
Share
Share
Download PDF
More article options
Visits
5072
Vol. 19. Issue 1.
Pages 82-84 (January - February 2015)
Brief communication
Open Access
Endocarditis by Kocuria rosea in an immunocompetent child
Visits
5072
Jorge Salomão Moreiraa, Adriana Gut Lopes Riccettob,
Corresponding author
aglriccetto@gmail.com

Corresponding author at: Center for Investigation in Pediatrics, Pediatrics Department, Faculty of Medical Sciences, State University of Campinas/Unicamp, Rua Tessália Vieira de Camargo, 126, Campinas, São Paulo CEP 13083-887, Brazil.
, Marcos Tadeu Nolasco da Silvab, Maria Marluce dos Santos Vilelab, Study Group Centro Médico de Campinas/Franceschi Medicina Laboratorial 1
a Pontificia Universidade Católica de Campinas/Puccamp, Campinas, SP, Brazil
b Pediatric Immunology, Center for Investigation in Pediatrics (CIPED), Pediatrics Department, Faculty of Medical Sciences, State University of Campinas/Unicamp, Campinas, SP, Brazil
This item has received

Under a Creative Commons license
Article information
Abstract
Full Text
Bibliography
Download PDF
Statistics
Tables (1)
Table 1. Reported cases of Kocuria sp infections in pediatric patients.
Abstract

Kocuria rosea belongs to genus Kocuria (Micrococcaceae family, suborder Micrococcineae, order Actinomycetales) that includes about 11 species of bacteria. Usually, Kocuria sp are commensal organisms that colonize oropharynx, skin and mucous membrane; Kocuria sp infections have been described in the last decade commonly affecting immunocompromised patients, using intravenous catheter or peritoneal dialysis. These patients had mainly bacteremia/recurrent sepsis. We hereby describe the case of a 10-year-old girl, immunocompetent, who had endocarditis/sepsis by K. rosea which was identified in five different blood cultures by Vitek 2 ID-GPC card (BioMérieux, France). Negative HIV serology, blood count within normal range of leukocytes/neutrophils and lymphocytes, normal fractions of the complement, normal level of immunoglobulins for the age; lymphocyte immunophenotyping was also within the expected values. Thymus image was normal at chest MRI. No catheters were required. Identification of K. rosea was essential to this case, allowing the differentiation of coagulase-negative staphylococci and use of an effective antibiotic treatment. Careful laboratory analysis of Gram-positive blood-born infections may reveal more cases of Kocuria sp infections in immunocompetent patients, which may collaborate for a better understanding, prevention and early treatment of these infections in pediatrics.

Keywords:
Infectious endocarditis
Kocuria rosea
Immune system
Children
Full Text

Kocuria rosea belongs to genus Kocuria (Micrococcaceae family, suborder Micrococcineae, order Actinomycetales) that includes about 11 species of bacteria, characteristically gram positive and aerobic (although some species like Kocuria kristinae, Kocuria marina and Kocuria rhizophila may proliferate in anaerobic conditions).1

Usually, Kocuria sp are commensal organisms that colonize oropharynx, skin, and mucous membrane; Kocuria sp infections have been described in the last decade commonly affecting immunocompromised patients, using intravenous catheter or peritoneal dialysis. These patients had mainly bacteremia/recurrent sepsis.1–5 It is noticeable, however, that the immunocompromise was not mandatory in all reported cases.3

Most pediatric cases were caused by K. kristinae; K. rosea was only described in one child until now, with peritonitis2–7 (Table 1).

Table 1.

Reported cases of Kocuria sp infections in pediatric patients.

Author/Year  Patient/Age  Clinical presentation  Etiology  Underlying condition 
Present case 2014  Female10 years old  Endocarditis  K. rosea  Congenital heart disease (Aortic coarctation/early surgical correction) 
Chen 2013  12 infants(0.6–3.3 months)  Apnea, bradicardia, desaturation, thrombocytosis, neutropenic fever, high fever  K. kristinae  6 – prematurity; 1 – acute leukemia (all 7 with central venous catheter); 5 without underlying diseases (with peripheral catheter) 
Dotis 2012  Female8 years old  Peritonitis  K. rosea  Peritoneal dialysis/dysplastic kidneys 
Moissenet 2012  Female3 years old  Persistent bloodstream infection  K. rhizophila  Total colonic form of Hirschsprung's disease/Terminal ileostomy and colostomy/Subcutaneous implantable vascular-access port for home parenteral nutrition 
Karadag 2012  Female4 months-old  Bloodstream infection and Black hairy tongue.  K. kristinae  Prolonged diarrhea and Severe failure to thrive. Total parenteral nutrition via a central venous catheter 
Lai 2010  Male2 years-old  Bloodstream infection  K. kristinae  Congenital short bowel Syndrome, hypogammaglobulinemia, Porth-A-cath for total parenteral nutrition 
Becker 2008  Male8 years old  Repeated septic episodes  K. kristinae  Methylmalonic aciduria due to a noncobalamin-responsive deficiency of methylmalonyl coenzyme A mutase. Subcutaneous implantable vascular-access port (Port-A-Cath; Vital-Port) for intravenous diet 

Laboratory identification of Kocuria sp by biochemistry methods is difficult due to similarity with other pathogens, especially coagulase-negative staphylococci, which delays the proper treatment.1,3

We herein describe a case of a 10-year-old girl who was diagnosed with aortic coarctation, which was surgically corrected at the age of 11 days. Since then she has had compensated congestive heart failure using propranolol and furosemide. This girl had appropriate weight and height for her age and no other co-morbidity during her life. At the age of 10 years she began having daily fever (without identified cause) and splenomegaly. Thirty days after the first fever episode, she had clinical and radiological diagnosis of pneumonia, at first treated with oral clarithromycin. After one week of treatment, the patient had again fever associated with headache, heart failure, and signs of sepsis; complementary imaging evaluation showed subarachnoid hemorrhage and bacterial endocarditis (vegetation in the mitral valve). During this period, five blood cultures, in three different days, were positive for K. rosea. The patient responded well to intravenous amoxicillin and clavulanate and support measures (oxygen by mask, diuretics); central intravenous catheter or other invasive procedures were not required.

As Kocuria sp infection is classically related to immunodeficiency, a specific investigation was carried out that negative for HIV infection, leukocytes/neutrophils and lymphocytes within normal range, normal fractions of the complement, normal levels of immunoglobulins for the age; lymphocyte immunophenotyping was also within the expected values. The thymus looked normal at chest MRI.

Kocuria sp laboratory identification was performed in a three-phase blood sample system (Probac); five samples, on three different days (day 0, 3 and 28), were positive for Gram-positive cocci in tetrads; the colony grew under aerobic conditions at 37°C; replication took place in 5% sheep blood medium (BioMérieux, France). Kocuria sp identification was performed by Vitek 2 ID-GPC card (BioMérieux, France). Analysis of the genome through molecular methods is desirable, but due to economic and technical limitations its use was not possible in our service.

Infective endocarditis (IE) is a disease with high mortality rate, despite medical advances. IE is uncommon in children under 17-year-old and most cases are associated with structural heart defects. A recent Canadian study involving 136 children with IE showed that cyanotic congenital cardiopathy and surgical correction before six months old were major risk factors.8 In the case shown here, both situations were present. The most common etiological agents for IE are Streptococcus viridans, Staphylococcus aureus, coagulase-negative Staphylococcus, and Streptococcus pneumoniae. Enterococcus and other Gram-negative are rare. The genus Kocuria is considered an atypical cause of endocarditis1; one case of IE by K. rosea was described in a 35-year-old woman, but no cases have been described in children.9 Regarding antibiotic susceptibility, Kocuria sp is sensitive to a variety of drugs (amoxicillin, cephalosporin, aminoglycoside, vancomycin, clindamycin); variable sensitivity to quinolones and sulfa.3,9 Amoxicillin-clavulanate has been proposed as the initial antibiotic treatment,1 as done in this case. Potentially contaminated catheters, if present, must be removed.

Many aspects of Kocuria sp infections are not yet entirely understood; besides human and other mammals, these bacteria may be found in drinking water sources, different sediments, seed and fermented food, being notable for its tropism for plastics. Kocuria sp usually form a biofilm, frequently in association with other bacteria.2 A recently identified K. rosea strain (BS1) is capable of producing an exopolysaccharide (called Kocuran), that has, in vitro, antioxidant and immunosuppressive properties – in human polymorphonuclear cultures stimulated with PHA, Kocuran inhibit the proliferation of these cells and also inhibit complement mediated hemolysis.10

There are few sporadic reports of Kocuria sp infections (especially by K. rosea); in the present case, despite IE risk factors, the lab screening for primary immunodeficiency was negative and there was no prolonged use of any kind of catheters. Genomic methods, as 16S RNA gene sequence, are desirable for correct identification of coagulase-negative staphylococci which presents a large phenotypic variation; this kind of approach is equally useful to confirm Kocuria species.11 However, despite not having used genomic methods and some restriction about Vitek 2 ID GPC card,11,12 identification of K. rosea was essential in this case, and subsequent use of effective antibiotic treatment. Careful laboratory analysis of Gram-positive blood infection may reveal more cases of Kocuria sp infections in immunocompetent patients, which may contribute for better understanding, prevention, and early treatment of these infections in pediatrics.

Conflicts of interest

The authors declare no conflicts of interest.

References
[1]
V. Savini, C. Catavitello, G. Masciarelli, et al.
Drug sensitivity and clinical impact of members of the genus Kocuria.
J Med Microbiol, 59 (2010), pp. 1395-1402
[2]
K. Becker, F. Rutsch, A. Uekötter, et al.
Kocuria rhizophila adds to the emerging spectrum of micrococcal species involved in human infections.
J Clin Microbiol, 46 (2008), pp. 3537-3539
[3]
J. Dotis, N. Printza, F. Papachristou.
Peritonitis attributable to Kocuria rosea in a pediatric peritoneal dialysis patient.
Perit Dial Int, 32 (2012), pp. 577-578
[4]
D. Moissenet, K. Becker, A. Mérens, et al.
Persistent bloodstream infection with Kocuria rhizophila related to a damaged central catheter.
J Clin Microbiol, 50 (2012), pp. 1495-1498
[5]
H.M. Chen, H. Chi, N.C. Chiu, et al.
Kocuria kristinae: a true pathogen in pediatric patients.
J Microbiol Immunol Infect, (2013),
[6]
E. Karadag Oncel, M.S. Boyraz, A. Kara.
Black tongue associated with Kocuria (Micrococcus) kristinae bacteremia in a 4-month-old infant.
Eur J Pediatr, 171 (2012), pp. 593
[7]
C.C. Lai, J.Y. Wang, S.H. Lin, et al.
Catheter-related bacteraemia and infective endocarditis caused by Kocuria species.
Clin Microbiol Infect, 17 (2011), pp. 190-192
[8]
D. Rushani, J.S. Kaufman, R. Ionescu-Ittu, et al.
Infective endocarditis in children with congenital heart disease: cumulative incidence and predictors.
Circulation, 128 (2013), pp. 1412-1419
[9]
K.H. Srinivasa, N. Agrawal, A. Agarwal, et al.
Dancing vegetations: Kocuria rosea endocarditis.
[10]
C.G. Kumar, P. Sujitha.
Kocuran an exopolysaccharide isolated from Kocuria rosea strain BS-1 and evaluation of its in vitro immunosuppression activities.
Enzyme Microb Technol, 55 (2014), pp. 113-120
[11]
R. Ben-Ami, S. Navon-Venezia, D. Schwartz, et al.
Erroneous reporting of coagulase-negative staphylococci as Kocuria spp. by the Vitek 2 system.
J Clin Microbiol, 43 (2005), pp. 1448-1450
[12]
M. Boudewijns, J. Vandeven, J. Verhaegen.
Vitek 2 automated identification system and Kocuria kristinae.
J Clin Microbiol, 43 (2005), pp. 5832

Address: Private Hospital, Centro Médico de Campinas and Private Laboratory Service, Franceschi Medicina Laboratorial, Campinas, SP, Brazil.

Copyright © 2014. Elsevier Editora Ltda.. All rights reserved
Download PDF
The Brazilian Journal of Infectious Diseases
Article options
Tools