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Vol. 16. Issue 3.
La Mucoviscidose
Pages 250-255 (May - June 2012)
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Vol. 16. Issue 3.
La Mucoviscidose
Pages 250-255 (May - June 2012)
Open Access
YMDD motif mutations in chronic hepatitis B antiviral treatment naïve patients: a multi-center study
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You-Wen Tana,
Corresponding author
tangyunn@yeah.net

Corresponding author at: The No. 3 People's Hospital of Zhenjiang, Zhenjiang 212000, Jiangsu, China.
, Guo-Hong Gea, Wei Zhaob, Jian-He Ganc, Yun Zhaod, Zhi-Lin Niue, Dong-Jun Zhangf, Li Chena, Xue- Jun Yua, Li-Jun Yanga
a Department of Liver Diseases, The No. 3 People's Hospital of Zhenjiang, Zhenjiang, China
b Department of Infectious Diseases, The No. 2 Hospital of Nanjin, Nanjing, China
c Department of Infectious Diseases, The First Affiliated Hospital of Suzhou University, Suzhou, China
d Department of Infectious Diseases, The Affiliated Hospital of Yangzhou University, Yangzhou, China
e Department of Infectious Diseases, The People's Hospital of Wujiang, Nantong University School of Medicine, Suzhou, China
f Department of Infectious Diseases, The People's Hospital of Danyang, Nantong University School of Medicine, Jiangsu, China
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Abstract
Objective

This study aimed to determine the natural prevalence of variants of tyrosinemethionine- aspartic acid-aspartic acid (YMDD) motif in patients with chronic hepatitis B (CHB), and to explore its relation with demographic and clinical features, hepatitis B virus (HBV) genotypes, and HBV DNA levels.

Methods

A total of 1,042 antiviral treatment naïve CHB patients (including with lamivudine [LAM]) in the past year were recruited from outpatient and inpatient departments of six centers from December 2008 to June 2010. YMDD variants were analyzed using the HBV drug resistance line probe assay (Inno-Lipa HBV-DR). HBV genotypes were detected with polymerase chain reaction (PCR) microcosmic nucleic acid cross-ELISA, and HBV deoxyribonucleic acid (DNA) was quantitated with real-time PCR. All serum samples underwent tests for HBV, HCV, and HDV with ELISA.

Results

YMDD variants were detected in 23.3% (243/1042) of CHB patients. YMDD mutation was accompanied by L180M mutation in 154 (76.9%) patients. Both wild-type HBV and YMDD variant HBV were present in 231 of 243 patients. Interestingly, 12 patients had only YIDD and/or YVDD variants without wild YMDD motif. In addition, 27.2% (98/359) of HbeAg-positive patients had YMDD mutations, which was higher than that in HbeAg-negative patients (21.2%, 145/683). The incidence of YMDD varied among patients with different HBV genotypes, but the difference was not significant. Moreover, the incidence of YMDD in patients with high HBV DNA level was significantly higher than that in those with low HBV DNA level.

Conclusion

Mutation of YMDD motif was detectable at a high rate in CHB patients in this study. The incidence of YMDD may be correlated with HBeAg and HBV DNA level.

Keywords:
YMDD
Mutations
Chronic hepatitis B
Anti-viral therapy
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