Journal Information
Vol. 16. Issue 1.
Pages 82-85 (January - February 2012)
Share
Share
Download PDF
More article options
Vol. 16. Issue 1.
Pages 82-85 (January - February 2012)
Open Access
Prevalence of antibodies to Borrelia burgdorferi sensu stricto in humans from a Cuban village
Visits
3473
Islay Rodrígueza,
Corresponding author
islay@ipk.sld.cu

Corresponding author at: Instituto de Medicina Tropical “Pedro Kourí”, Novia del Mediodía, km 6 1/2 – 00, La Lisa, Havana, Cuba.
, Carmen Fernándeza, Lizet Sáncheza, Bárbara Martínezb, Hans H. Siegristc, Reto Lienhardc
a Tropical Medicine Institute “Pedro Kourí”, Havana, Cuba
b Medical Assistant Centre of Sierra del Rosario, Artemisa, Cuba
c Laboratory of Microbiology, ADMed, La Chaux-de-Fonds, Switzerland
This item has received

Under a Creative Commons license
Article information
Abstract

Lyme disease has not been officially reported in Cuba. However, clinical cases have been serologically reported. Seroprevalence survey of Borrelia burgdorferi sensu stricto antibodies in humans in the country has not been conducted.

Objective

To estimate the prevalence of borrelial antibodies in inhabitants of a village with historically high level of tick infestation.

Methods

Serum specimens from 247 persons randomly selected from the population of the village were examined by IgG Western blot using B31 strain for estimating the prevalence of antibodies profile.

Results

A seroprevalence value interval (95% CI) of 0.6%–7.2% was estimated for the studied population. The prevalent borrelial protein bands on immunoblots were 41, 72, 90/93, 34, 47, 60, 58, 56, 65/66 and 31 kDa in a decreasing order of significance.

Conclusion

These results support the previous serological findings, suggesting the presence of this borreliosis in Cuba.

Keywords:
Borrelia burgdorferi
Prevalence
Lyme disease
Antibodies
Full text is only aviable in PDF
References
[1.]
N. Rudenko, M.L. Grubhoffer, J.H. Oliver.
Borrelia carolinensis sp. nov., a new (14th) member of the Borrelia burgdorferi sensu lato complex from the southeastern region of the United States.
J Clin Microbiol, 47 (2009), pp. 134-141
[2.]
N. Rudenko, M. Golovchenko, T. Lin, et al.
Delineation of a new species of the Borrelia burgdorferi sensu lato complex Borrelia americana sp. nov.
J Clin Microbiol, 47 (2009), pp. 3875-3880
[3.]
G. Stanek, M. Reiter.
The expanding Lyme Borrelia complex-clinical significance of genomic species?.
Clin Microbiol Infect, 17 (2011), pp. 487-493
[4.]
R.L. Bratton, J.W. Whiteside, M.J. Hovan, et al.
Diagnosis and treatment of Lyme disease.
Mayo Clin Proc, 83 (2008), pp. 566-571
[5.]
B. Wilske, V. Fingerle, U. Schulte-Spechtel.
Microbiological and serological diagnosis of Lyme borreliosis.
FEMS Immunol Med Microbiol, 49 (2007), pp. 13-21
[6.]
I. Rodríguez, R. Pedroso, C. Fernández, M. Cinco, O. Fuentes.
Lyme disease in Cuba? Report of possible cases.
Rev Cubana Med Trop, 55 (2003), pp. 41-43
[7.]
I. Rodríguez, C. Fernández, M. Cinco, et al.
Do antiborrelial antibodies suggest Lyme disease in Cuba?.
Emerg Infect Dis, 10 (2004), pp. 1698-1700
[8.]
I. Rodríguez, L.M. Ortega, C. Fernández, et al.
Lyme borreliosis in Cuba Based on new cases.
Rev Panam Infect, 11 (2009), pp. 37-41
[9.]
Herrera M, García M (active August 2009). UNESCO Work document Nu. 10: Sierra del Rosario Biosphere Reserve (Cuba); 1995: http://unesdoc.unesco.org/images/0011/001139/113934so.pdf
[10.]
S. Mavin, R.M. Milner, R. Evans, et al.
The use of local isolates in Western blots improves serological diagnosis of Lyme disease in Scotland.
J Med Microbiol, 56 (2007), pp. 47-51
[11.]
Centers for Disease Control and Prevention.
Recommendations for test performance and interpretation from the Second National Conference on Serologic Diagnosis of Lyme Disease.
Morbid Mortal Wkly Rep, 44 (1995), pp. 590-591
[12.]
E. Hilton, J. Devoti, S. Sood.
Recommendation to include OspA and OspB in the new immunoblotting criteria for serodiagnosis of Lyme disease.
J Clin Microbiol, 34 (1996), pp. 1353-1354
[13.]
H. Bruckbauer, V. Preac-Mursic, R. Fuchs, et al.
Cross-reactive proteins of Borrelia burgdorferi.
Eur J Clin Microbiol Infect Dis, 11 (1992), pp. 1-9
[14.]
G. Gordillo, J. Torres, F. Solorzano, et al.
Serologic evidences suggesting the presence of Borrelia burgdorferi infection in Mexico.
Arch Med Res, 30 (1999), pp. 64-68
[15.]
G. Gordillo-Pérez, J. Torres, F. Solórzano-Santos, et al.
Seroepidemiologic study of Lyme's borreliosis in Mexico City and the northeast of the Mexican Republic.
Salud Publica Mex, 45 (2003), pp. 351-355
[16.]
A. Hristea, S. Hristescu, C. Ciufecu, et al.
Seroprevalence of Borrelia burgdorferi in Romania.
Eur J Epidemiol, 17 (2001), pp. 891-896
[17.]
A.D. Kaya, A.H. Parlak, C.E. Ozturk, et al.
Seroprevalence of Borrelia burgdorferi infection among forestry workers and farmers in Duzce, north-western Turkey.
New Microbiol, 31 (2008), pp. 203-209
[18.]
P. Tomao, L. Ciceroni, M.C. D’Ovidio, et al.
Prevalence and incidence of antibodies to Borrelia burgdorferi and to tick-borne encephalitis virus in agricultural and forestry workers from Tuscany, Italy.
Eur J Clin Microbiol Infect Dis, 24 (2005), pp. 457-463
[19.]
M. Cinco, F. Barbone, M. Grazia-Ciufolini, et al.
Seroprevalence of tick-borne infections in forestry rangers from northeastern Italy.
Clin Microbiol Infect, 10 (2004), pp. 1056-1061
[20.]
J.A. Oteo, V. Martinez de Artola, J. Casas, et al.
Epidemiology and prevalence of seropositivity against Borrelia burgdorferi antigen in La Rioja, Spain.
Rev Epidemiol Sante Publique, 40 (1992), pp. 85-92
[21.]
S. Bazovska, E. Machakova, M. Spalekova, et al.
Reported incidence of Lyme disease in Slovakia and antibodies to B. burgdorferi antigens detected in healthy population.
Bratisl Lek Listy, 106 (2005), pp. 270-273
[22.]
J. Robertson, E. Guy, N. Andrews, et al.
A European multicenter study of immunoblotting in serodiagnosis of Lyme borreliosis.
J Clin Microbiol, 38 (2000), pp. 2097-2102
[23.]
S.M. Engstrom, E. Shoop, R. Johnson.
Immunoblot interpretation criteria for serodiagnosis of early Lyme disease.
J Clin Microbiol, 33 (1995), pp. 419-427
[24.]
K. Tilly, P.A. Rosa, P.E. Stewart.
Biology of infection with Borrelia burgdorferi.
Infect Dis Clin N Am, 22 (2008), pp. 217-234
[25.]
J. Gutiérrez, M. Guerrero, F. Nuñez, et al.
Antibodies to Borrelia burgdorferi in European populations.
J Clin Lab Anal, 14 (2000), pp. 20-26
Copyright © 2012. Elsevier Editora Ltda.. All rights reserved
Download PDF
The Brazilian Journal of Infectious Diseases
Article options
Tools