Journal Information
Vol. 15. Issue 6.
Pages 528-532 (November - December 2011)
Share
Share
Download PDF
More article options
Vol. 15. Issue 6.
Pages 528-532 (November - December 2011)
Original article
Open Access
Evaluation of the presence of microorganisms in solid-organ preservation solution
Visits
2873
André Marcelo Colvara Mattana1, Alexandre Rodrigues Marra2, Antônia Maria de Oliveira Machado3, Gaspar de Jesus Lopes Filho4, Alcides Augusto Salzedas Netto5, Adriano Miziara Gonzalez6,
Corresponding author
amgonzalez@uol.com.br

Correspondence to: Rua Napoleão de Barros, 610 Vila Clementino, 04024-002, São Paulo, SP Brazil, Phone: +55 11 5084-7551/5084-9131.
1 Postgraduate Student, Surgical Gastroenterology Service, Universidade Federal de São Paulo – Escola Paulista de Medicina (UNIFESP-EPM); General and Digestive Surgeon, Hospital Santo Antônio and Hospital Santa Catarina, Blumenal, SC, Brazil
2 Infectologist and Intensivist, Hospital Israelita Albert Einstein, SP, Brazil
3 Technical Director, Instituto Paulista de Doenças Infecciosas e Parasitárias; Technical Director, Laboratório Central do Hospital São Paulo, SP, Brazil
4 UNIFESP; Chief of Surgical Gastroenterology, Universidade Estadual Paulista (UNESP), SP, Brazil
5 Surgeon, Kidney Transplantation Program, Hospital do Câncer and Hospital Sírio-Libanês; Assistant of Pediatric Surgery, UNIFESP-EPM, SP, Brazil
6 Digestive Transplantation Coordinator, UNIFESP, SP, Brazil
This item has received

Under a Creative Commons license
Article information
Abstract
Objective

To assess the presence of microorganism contamination in the preservation solution for transplant organs (kidney/pancreas).

Method

Between August 2007 and March 2008, 136 samples of preservation solution were studied prior to graft implantation. Variables related to the donor and to the presence of microorganisms in the preservation solution of organs were evaluated, after which the contamination was evaluated in relation to the “recipient culture” variable. Univariate and multivariate statistical analyses were performed.

Results

The contamination rate of the preservation solution was 27.9%. Coagulase-negative Staphylococcus was the most frequently isolated microorganism. However, highly virulent agents, such as fungi and enterobacteria, were also isolated. In univariate analysis, the variable “donor antibiotic use” was significantly associated to the contamination of the preservation solution. On the other hand, multivariate analysis found statistical significance in “donor antibiotic use” and “donor's infectious complications” variables.

Conclusions

In this study, 27.9% of the preservation solutions of transplant organs were contaminated. Infectious diseases and non-use of antibiotics by the donor were significantly related to the presence of microorganisms in organ preservation solutions. Contamination in organ preservation solutions was not associated with infection in the recipient.

Keywords:
organ transplantation
disease transmission, infectious
organ preservation solutions
microbial viability
Full text is only aviable in PDF
References
[1.]
Marinho A. Um estudo sobre as filas para transplantes no Sístema Único de Saúde brasileiro Cad Saúde Pública. 22[10], 2229-2239. 2006.
[2.]
P. Bucher, Z. Mathe, D. Bosco, et al.
Microbial surveillance during human pancreatic islet isolation.
Transplant Proc, 36 (2004), pp. 1147-1148
[3.]
J.A. Majeski, J.W. Alexander, M.R. First, R. Munda, J.P. Fidler, T.K. Craycraft.
Transplantation of microbially contaminated cadaver kidneys.
Arch Surg, 117 (1982), pp. 221-224
[4.]
S.J. Wakelin, J. Casey, A. Robertson, et al.
The incidence and importance of bacterial contaminants of cadaveric renal perfusion fluid.
Transpl Int, 17 (2005), pp. 680-686
[5.]
E. Cerutti, C. Stratta, R. Romagnoli, R. Serra.
Bacterial- and fungalpositive cultures in organ donors: clinical impact in liver transplantation.
Liver Transpl, 12 (2006), pp. 1253-1259
[6.]
D.R. Cox.
Regression models life-tables.
J R Stat Soc Ser B, 34 (1972), pp. 187-220
[7.]
W.J. Couto, A.C. Carvalho, J.N.R. Branco, et al.
Transplante cardíaco e infecção.
Bras Cir Cardiovasc, 16 (2001), pp. 141-151
[8.]
G.C. McCoy, S. Loening, W.E. Braun, M.O. Magnusson, L.H. Banowsky, M.C. McHenry.
The fate of cadaver renal allografts contaminated before transplantation.
Transplantation, 20 (1975), pp. 467-472
[9.]
D.E. Lammermeier, M.S. Sweeney, H.E. Haupt, B. Radovancevic, J.M. Duncan, O.H. Frazier.
Use of potentially infected donor hearts for cardiac transplantation.
Ann Thorac Surg, 50 (1990), pp. 225
[10.]
R.P. Wood, T. Melendez, A.N. Langnas, H. Navakal, D. Kelly.
The viability of microorganisms in preservation solutions.
Transplantation, 51 (1991), pp. 239-242
[11.]
D.R. Snydman.
Infection in solid organ transplantation.
Transpl Infect Dis, 1 (1999), pp. 21-28
[12.]
N.M. Rogers, C.A. Peh, R. Faull, M. Pannell, J. Cooper, G.R. Russ.
Transmission of toxoplasmosis in two renal allograft recipients receiving an organ from the same donor.
Transpl Infect Dis, 10 (2008), pp. 71-74
[13.]
M.M. Linhares, A.M. Gonzalez, T. Triviño, et al.
Simultaneous pancreas-kidney transplantation: infectious complications and microbiological aspects.
Transplant Proc, 36 (2004), pp. 980-981
[14.]
D.M. Little, J.G. Farrell, P.M. Cunningham, D.P. Hickey.
Donor sepsis is not a contraindication to cadaveric organ donation.
Q J Med, 90 (1997), pp. 641-642
[15.]
M. Haberal, A. Demirag, B. Cohen, M. Turan, G. Arslan, N. Bilgin.
Microbiologic analysis of preservation solutions in cadaveric kidney transplantation.
Transplant Proc, 27 (1995), pp. 2697-2698
[16.]
K.M. Gottesdiener.
Transplanted infections: donor-to-host transmission with the allograft.
Ann Intern Med, 110 (1989), pp. 1001-1016
[17.]
F.L. Delmonico, D.R. Snydman.
Organ donor screening for infectious diseases: review of practice and implications for transplantation.
Transplantation, 65 (1998), pp. 603-610
[18.]
F. Mattner, S. Fischer, A. Kola, et al.
Impact of bacterial and fungal donor organ contamination in lung, heart-lung, heart and liver transplantation.
Infection, 36 (2008), pp. 207-212
[19.]
G.B. Zibari, J. Lipka, H. Zizzi, K.D. Abreo, L. Jacobbi, J.C. McDonald.
The use of contaminated donor organs in transplantation.
Clin Transplantation, 14 (2000), pp. 397-400
[20.]
O. Len, J. Gavalda, M. Blanes, et al.
Donor infection and transmission to the recipient of a solid allograft.
Am J Transplant, 8 (2008), pp. 2420-2425
Copyright © 2011. Elsevier Editora Ltda.. All rights reserved
Download PDF
The Brazilian Journal of Infectious Diseases
Article options
Tools