Article information
Abstract
A mutation described as a G-to-A transition has been reported in SDF-1 gene (SDF1-3’A), being prevalent in all ethnic groups, except in Africans. This mutation is associated with the onset of AIDS progression. Our aim was to identify the frequency of this allele in different groups from Brazil: Tiriyó and Waiampi Amerindian tribes (Asian ancestry); selected blood donors from Joinville (German descendents); and from Salvador (predominance of African and Portuguese mixture). SDF1-3’A was screened by PCR/RFLP with MspI enzyme. Our results showed a high allelic frequency in Tiriyó tribe (0.24) and Joinville population (0.21), and a frequency of 0.17 and 0.05 in the Salvador population and in the Waiampi tribe, respectively. There was no statistical difference among the allelic frequencies in the studied ethnic groups, except in the Waiampi. Due to the great genetic diversity among Brazilian population and the lack of studies on SDF1-3’A allele, our study of this allelic frequency in these different Brazilian ethnic groups could be important to identification of biomarker for therapeutic support in progression to AIDS and a molecular marker for analysis of evolutionary relationships among human populations.
Keywords:
SDF1-3’A polymorphism
brazilian population
HIV-1 co-receptor
Full text is only aviable in PDF
References
[1.]
H. Deng, R. Liu, W. Ellmeier, et al.
Identification of a major coreceptor for primary isolates of HIV-1.
Nature, 381 (1996), pp. 661-666
[2.]
S. Mummidi, S.S. Ahuja, E. Gonzalez, et al.
Genealogy of the CCR5 locus and chemokine system gene variants associated with altered rates of HIV-1 disease progression.
Nat. Med., 4 (1998), pp. 786-793
[3.]
E.A. Berger, P.M. Murphy, J.M. Farber.
Chemokine receptors as HIV-1 coreceptors: roles in viral entry, tropism, and disease.
Annu. Rev. Immunol., 17 (1999), pp. 657-700
[4.]
T. Dragic, V. Litwin, G.P. Allaway, et al.
HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5.
Nature, 381 (1996), pp. 667-673
[5.]
R. Grimaldi, N. Shindo, X. Acosta, et al.
Prevalence of the CCR5Delta32 mutation in Brazilian populations and cell susceptibility to HIV-1 infection.
Hum Genet., 111 (2002), pp. 102-104
[6.]
H. Liu, Y. Hwangbo, S. Holte, et al.
Analysis of genetic polymorphisms in CCR5, CCR2, stromal cell-derived factor-1, RANTES, and dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin in seronegative individuals repeatedly exposed to HIV-1.
J Infect Dis., 190 (2004), pp. 1055-1058
[7.]
W.S. Modi, K. Scott, J.J. Goedert, et al.
Haplotype analysis of the SDF-1 (CXCL12) gene in a longitudinal HIV-1/AIDS cohort study.
Genes Immun., 6 (2005), pp. 691-698
[8.]
S. Sei, A.M. Boler, G.T. Nguyen, et al.
Protective effect of CCR5 delta 32 heterozygosity is restricted by SDF-1 genotype in children with HIV-1 infection.
AIDS, 15 (2001), pp. 1343-1352
[9.]
C. Winkler, W. Modi, M.W. Smith, et al.
Genetic restriction of AIDS pathogenesis by an SDF-1 chemokine gene variant. ALIVE Study, Hemophilia Growth and Development Study (HGDS), Multicenter AIDS Cohort Study (MACS), Multicenter Hemophilia Cohort Study (MHCS), San Francisco City Cohort (SFCC).
Science, 279 (1998), pp. 389-393
[10.]
C.S. Dezzutti, P.C. Guenthner, T.A. Green, O.J. Cohen, T.J. Spira, R.B. Lal.
Stromal-derived factor-1 chemokine gene variant is associated with the delay of HIV-1 disease progression in two longitudinal cohorts.
AIDS, 14 (2000), pp. 894-896
[11.]
L.L. Cavalli-Sforza, M.W. Feldman.
The application of molecular genetic approaches to the study of human evolution.
Nat Genet., 33 (2003), pp. 266-275
[12.]
M. Bamshad, S. Wooding, B.A. Salisbury, J.C. Stephens.
Deconstructing the relationship between genetics and race.
Nat Rev Genet., 5 (2004), pp. 598-609
[13.]
N.A. Rosenberg, S. Mahajan, S. Ramachandran, C. Zhao, J.K. Pritchard, M.W. Feldman.
Clines, clusters, and the effect of study design on the inference of human population structure.
PLoS Genet., 1 (2005), pp. e70
[14.]
IBGE-Instituto Brasileiro de Geografia e Estatística 2000. Pesquisa Nacional de Amostragem por Domicílio – Censo. http://www.ibge.gov.br
[15.]
E.S. Azevedo, C.M.M. Fortuna, K.M.C. Silva.
Spread and diversity of human population in Bahia, Brazil.
Hum. Biol., 54 (1982), pp. 329-341
[16.]
I. Dourado, M.L. Barreto, M.G. Teixeira, B. Galvão-Castro.
HTLV-I in the general population of a Brazilian city with the highest seroprevalence.
AIDS. Res. Hum. Retrov., 17 (2001),
[17.]
M.W. Smith, M. Carrington, C. Winkler, et al.
CCR2 chemokine receptor and AIDS progression.
Nat Med., 3 (1997), pp. 1052-1053
[18.]
R.P. van Rij, S. Broersen, J. Goudsmit, R.A. Coutinho, H. Schuitemaker.
The role of a stromal cell-derived factor-1 chemokine gene variant in the clinical course of HIV-1 infection.
AIDS, 12 (1998), pp. F85-F90
[19.]
F. Vidal, J. Peraire, P. Domingo, et al.
Chemokines and Long-Term Nonprogressor Study Group. Lack of association of SDF-1 3A variant allele with long-term nonprogressive HIV-1 infection is extended beyond 16 years.
J Acquir Immune Defic Syndr., 40 (2005), pp. 276-279
[20.]
M.A. Watanabe, G.G. de Oliveira Cavassin, M.D. Orellana, et al.
SDF-1 gene polymorphisms and syncytia induction in Brazilian HIV-1 infected individuals.
Microb. Pathog., 35 (2003), pp. 31-34
[21.]
De Pinho Lott Carvalhaes FA, Cardoso GL, Hamoy IG, Liu YT, Guerreiro JF. Distribution of CCR5-delta32, CCR2-64I, and SDF1-3A mutations in populations from the Brazilian Amazon region Hum Biol. 2004; Aug; 76(4):643-6.
[22.]
W.A. Silva Jr, S.L. Bonatto, A.J. Holanda, et al.
Mitochondrial genome diversity of Native Americans supports a single early entry of founder populations into America.
Am J Hum Genet., 71 (2002), pp. 187-192
[23.]
R.C. Mousinho-Ribeiro, G. Pante-de-Sousa, E.J. Melo dos Santos, J.F. Guerreiro.
Genetic relationships among native americans based on beta-globin gene cluster haplotype frequencies.
Genet. Mol. Biol., 26 (2003), pp. 229-234
[24.]
S. Wang, C.M. Lewis Jr, M. Jakobsson, S. Ramachandran, N. Ray.
Genetic Variation and Population Structure in Native Americans.
PLoS Genet., 3 (2007), pp. e185
Copyright © 2010. Elsevier Editora Ltda.. All rights reserved
