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Vol. 23. Issue 2.
Pages 146-147 (March - April 2019)
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Vol. 23. Issue 2.
Pages 146-147 (March - April 2019)
Letter to the editor
DOI: 10.1016/j.bjid.2019.04.007
Open Access
Occurrence of hemoplasmas in free-ranging and captive wild felines
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Letícia Camara Pitchenin
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leticia_lecamara@hotmail.com

Corresponding author.
, Fernanda Harumi Maruyama, Thais de Oliveira Morgado, Valeria Dutra, Luciano Nakazato
Federal University of Mato Grosso, Department of Veterinary Science, Postgraduate Course in Veterinary Science, Cuiabá, Mato Grosso, Brazil
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Table 1. PCR results of hemoplasmas according to species and habitat.
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Dear Editor:

Hemotropic mycoplasmas or hemoplasmas are defined as bacteria of reduced size and genome, which lack a cell wall. They are associated with the cytoplasmic membrane of erythrocytes in several hosts. In felines, three significant species are described: Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum, and Candidatus Mycoplasma turicensis.1

Infected animals show illness of varying degrees, ranging from asymptomatic anemia to severe hemolytic attacks. The presence of compounding pathologies, individual susceptibility, and species of hemoplasma involved contribute to variation in the clinical presentation of feline hemoplasmosis.1

Few studies report the occurrence of these microorganisms in wild felines, particularly in Brazil,2 and reports about free-ranging animals are even more rare. This study aimed to determine the prevalence of the three clinically relevant hemoplasma species in wild free-ranging and captive felines.

From August 2014 to August 2018, blood was collected from 23 wild felines according to “Sistema de Autorização e Informação em Biodiversidade” (SISBIO – nos. 40617-1 and 42303); their species and habitat are described in Table 1. Total blood DNA extraction of the samples was accomplished with a phenol/chloroform protocol.3 PCR was performed for each of the three microorganisms according to Makino et al.4 The product of a positive PCR was purified using GFX PCR DNA and a Gel Band Purification Kit (GE Healthcare UK Limited, Amersham, UK). It was then automatically sequenced (ABI 3500 Genetic Analyzer, Applied Biosystems, Foster City, USA) using the BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems). The sequence was compared to the GenBank database using the BLAST on the NCBI server (http: www.ncbi.nlm.nih.gov/BLAST).

Table 1.

PCR results of hemoplasmas according to species and habitat.

Identification  Species  Habitat  M. haemofelis  Candidatus M. haemominutum  Candidatus M. turicensis 
M941/14  Leopardus colocolo  Free-ranging  −  − 
M1446/15  Leopardus pardalis  Free-ranging  −  − 
M382/16  Leopardus pardalis  Captive  −  − 
M462/16  Leopardus pardalis  Free-ranging  −  −  − 
M953/14  Leopardus pardalis  Captive  − 
M1036/16  Leopardus pardalis  Captive  − 
M1037/16  Leopardus pardalis  Captive  −  − 
M1226/16  Leopardus pardalis  Free-ranging  −  − 
M982/18  Leopardus pardalis  Free-ranging  −  −  − 
M787/17  Leopardus wiedii  Captive  −  − 
M1242/18  Panthera onca  Captive  −  − 
M922/15  Panthera onca  Free-ranging  −  − 
M1013/16  Panthera onca  Free-ranging  −  − 
M1328/14  Puma concolor  Captive  −  −  − 
M1102/16  Puma concolor  Captive  −  −  − 
M474/15  Puma concolor  Free-ranging  −  − 
M849/16  Puma concolor  Captive  −  −  − 
M857/16  Puma concolor  Free-ranging  −  −  − 
M75/17  Puma concolor  Captive  −  −  − 
M1118/17  Puma concolor  Free-ranging  −  −  − 
M1309/17  Puma concolor  Free-ranging  −  −  − 
M937/18  Puma concolor  Free-ranging  −  −  − 
M609/15  Puma yagouaroundi  Free-ranging  −  − 
Total0 (0%)  3 (12.5%)  12 (52.2%) 

M. haemofelis was not detected in any sample. Candidatus M. haemominutum was detected in three felines (12.5%) of the species Leopardus pardalis; two animals were free-ranging and one was in captivity. Candidatus M. turicensis was present in 12 felines (52.2%): six free-ranging and six captive animals. All tested feline species showed at least one animal affected by the microorganism making this the first report on the occurrence of Candidatus M. turicensis in Leopardus colocolo, L. wiedii, Puma concolor, Panthera once, and Puma yagouaroundi.

Negative PCR results for M. haemofelis may not necessarily indicate that the cats were free of infection, considering the significant fluctuations of M. haemofelis presence in the blood.5

Candidatus M. haemominutum is the least pathogenic of these three species of hemoplasms. Wild felines are more resistant than domestic felines, and generally clear a Candidatus M. haemominutum infection after 1–2 years.2 This finding lends support to the low rate of detection in the animals we tested.

The detection of Candidatus M. turicensis DNA in more than 50% of free-ranging and captive animals demonstrates the broad distribution capacity of this agent. Fleas and ticks are vectors of many hemoplasmas. However, Candidatus M. turicensis is more closely related to rodent hemoplasmas, making the possibility of interspecies transmission plausible.5

Although none of the animals tested positive for M. haemofelis, there were a few detected cases of Candidatus M. haemominutum and a high occurrence of Candidatus M. turicensis.

Zoonotic hemoplasma infections are emerging, with an increasing number of reported cases, particularly in immunocompromised patients.2 This study in wild feline populations contributes to our understanding of the epidemiology of hemoplamosis and the importance of these animals as a source of infection in animal and human populations.

Disclaimers

The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the Centers for Disease Control and Prevention or the institutions with which the authors are affiliated.

Acknowledgment

The authors are grateful to CAPES for financial support through a scholarship.

References
[1]
J.E. Sykes.
Feline hemotropic mycoplasmas.
Vet Clin North Am Small Anim Pract, 40 (2010), pp. 1157-1170
[2]
C.M. Ribeiro, A.C. Matos, V.B. Richini-Pereira, et al.
Occurrence and phylogenetic analysis of ‘Candidatus Mycoplasma haemominutum’ in wild felines from Paraná, Brazil.
Semina Ciênc Agrár, 38 (2017), pp. 2837-2844
[3]
J. Sambrook, D.W. Russell.
Molecular cloning: a laboratory manual.
Cold Spring Harbor Laboratory Press, (2001), pp. 2100
[4]
H. Makino, D.A.J. Paula, V.R.F. Sousa, et al.
Natural hemoplasma infection of cats in Cuiaba Mato Grosso, Brazil.
Semina Ciênc Agrár, 39 (2018), pp. 875-880
[5]
B. Willi, C. Filoni, J.L. Catao-Dias, et al.
Worldwide occurrence of feline hemoplasma infections in wild felid species.
J Clin Microbiol, 45 (2007), pp. 1159-1166
Copyright © 2019. Sociedade Brasileira de Infectologia
The Brazilian Journal of Infectious Diseases

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