NotaIdentificación de levaduras del género Candida: los métodos convencionales frente a MALDI-TOF MSIdentification of Candida yeasts: Conventional methods and MALDI-TOF MS
Section snippets
Materiales y métodos
Se analizaron 197 aislamientos de Candida: 28 Candida parapsilosis, 34 Candida glabrata, 24 Candida krusei, 45 Candida tropicalis, 30 Candida guilliermondii, 28 C. albicans, 6 Candida dubliniensis, 1 Candida kefyr y 1 Candida lipolytica, provenientes de materiales clínicos; se incluyeron las cepas de la colección ATCC C. parapsilosis 22019, C. glabrata 90030, C. krusei 6258 y C. albicans 68548 (tabla 1). Todos los aislamientos fueron identificados por métodos convencionales y MALDI-TOF MS. Las
Resultados y discusión
La tabla 2 muestra los resultados obtenidos con ambas metodologías. El porcentaje de acuerdo o concordancia directa entre la identificación convencional y la espectrometría de masas (MALDI-TOF MS) fue del 92,5% (IC del 95%: 91,64-93,43) (186 aislamientos de 201), y un índice kappa igual a 0,751 (IC del 95%: 0,633-0,869), lo que indica una buena concordancia entre ambas técnicas diagnósticas. Se observaron discrepancias con las siguientes especies y número de aislamientos: C. parapsilosis
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Los componentes del grupo están relacionados en el anexo 1.