Rapid communicationProteome analysis of the causative agent of Chagas disease: Trypanosoma cruzi
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Acknowledgments
We thank Drs Samuel Goldenberg and Marco Krieger (Instituto de Biologia Molecular do Parana, Brazil) for Dm28c clone, and Magdalena Portela for technical assistance. This work was supported by CSIC-Universidad de la República (Montevideo, Uruguay), the AMSUD-Pasteur Program and ECOS-SUD Program (Uruguay-France).
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2015, Journal of ProteomicsCitation Excerpt :The presence of non-translated mRNA described in T. cruzi [14] provides strong evidence for the requirement of protein content analysis in differential gene expression studies in trypanosomatids models; therefore, large-scale proteomic technology could be an attractive alternative. The T. cruzi proteomic map was first assessed in 2004 [15], and in the last ten years, descriptive analyses were performed in a variety of evolutive stages and strains, and differential expression studies were also conducted to evaluate the parasite's susceptibility/resistance to drugs and its adaptation to stress conditions [10–12,15–24]. Specifically for trypomastigote samples, despite two studies that were performed employing two-dimensional electrophoresis (2-DE) followed by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF MS) [15,25], most of the proteomic analysis of this parasite form involved a liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC–ESI-MS/MS) approach.