TY - JOUR T1 - Preservation of cytotoxic granule production in response to mycobacterial antigens by T-lymphocytes from vertically HIV-infected Brazilian youth on effective combined antiretroviral therapy JO - The Brazilian Journal of Infectious Diseases T2 - AU - Pedromonico Arrym,Mauro AU - Martins Alves,Paulo César AU - Virginello Castelhano,Mariana AU - Nitsch Mazzola,Taís AU - Muller Banzato Pinto de Lemos,Renata AU - Zaccariotto,Tânia Regina AU - Levy,Carlos Emilio AU - Guimarães,Fernando AU - Nolasco da Silva,Marcos Tadeu SN - 14138670 M3 - 10.1016/j.bjid.2019.06.002 DO - 10.1016/j.bjid.2019.06.002 UR - https://bjid.org.br/en-preservation-cytotoxic-granule-production-in-articulo-S1413867018310523 AB - BackgroundHIV infection harms adaptive cellular immunity mechanisms. Long-term virological control by combined antiretroviral therapy (cART) reduces the risk of mycobacterial infections. Thus, we aimed to study cellular responses to mycobacterial antigens in 20 HIV-infected adolescents with at least one year of virological control (HIV-RNA <40 copies/mL) and 20 healthy adolescents. MethodsWe evaluated CD8 and γδ T-cell degranulation by measurement of CD107a membrane expression after stimulation with lysates from BCG (10 μg/mL) and H37RA Mycobacterium tuberculosis (Mtb, 10 μg/mL). Immune activation and antigen-presenting ability were also assessed by determination of HLA-DR, CD80, and CD86 markers. ResultsTCR γδ T-cell CD107a expression was similar between groups in response to mycobacterial antigens, and lower in the HIV-infected group in response to mitogen. Higher baseline HLA-DR expression and lower mycobacterial-stimulated expression was found within the HIV-infected group. ConclusionsSimilar degranulation in stimulated CD8+ and TCR γδ T-cells from HIV-infected adolescents, when compared to healthy controls suggests long-term immunological preservation with immune reconstitution under successful cART. However, differences in HLA-DR expression may represent ongoing inflammation and lower specific responses in HIV-infected youth. These features may be relevant in the context of the precocity and severity of vertically acquired HIV infection. ER -