TY - JOUR T1 - Papillomavirus infections in the oral and genital mucosa of asymptomatic women JO - The Brazilian Journal of Infectious Diseases T2 - AU - Oliveira,Ledy Horto Santos AU - Santos,Larissa Silva AU - Silva,Carolina Oliveira AU - Augusto,Everton Faccini AU - Neves,Felipe Piedade Gonçalves SN - 14138670 M3 - 10.1016/j.bjid.2016.08.015 DO - 10.1016/j.bjid.2016.08.015 UR - https://bjid.org.br/en-papillomavirus-infections-in-oral-genital-articulo-S1413867016304160 AB - Human papillomavirus (HPV) has been found in several regions of the body, including the oral cavity. Recently, this virus has been associated with oropharyngeal cancer, but little is known about HPV transmission to the oral cavity. We carried out a study to investigate concurrent oral and cervical infections in 76 asymptomatic women attending a healthcare program. Demographic and behavior data were obtained through a structured questionnaire. Oral and cervical mucosa scrapings were collected and stored for DNA extraction. HPV DNA amplification was performed by polymerase chain reaction assay (PCR) using both primers My09/My11 and FAP59/64, followed by HPV typing with restriction fragment length polymorphism analysis (RFLP) and sequencing. The data collected revealed no risk factors for HPV infection in these 76 women. HPV prevalence of 9.2 and 5.3% was found in cervical and oral mucosa, respectively. Concurrent infections by discordant types were detected in one case only. Sequencing procedures allowed us to detect a new putative HPV 17 subtype from the Betapapillomavirus genus. Our results support the view that cervical and oral HPV infections are independent events. The observed low prevalence of both oral and cervical HPV infections could be associated with attendance in a healthcare program. ER -