The Brazilian Journal of Infectious Diseases The Brazilian Journal of Infectious Diseases
Braz J Infect Dis 2017;21:418-23 - Vol. 21 Num.4 DOI: 10.1016/j.bjid.2017.04.002
Original article
Role of MyD88-adaptor-like gene polymorphism rs8177374 in modulation of malaria severity in the Pakistani population
Asima Rania,, , Syed Kashif Nawaza, Shazia Irfana, Muhammad Arshadb, Razia Bashira, Najma Shaheenc
a University of Sargodha, Department of Zoology, Sargodha, Pakistan
b University of Education, Lower Mall Campus, Lahore, Pakistan
c University of the Punjab, Department of Zoology, Lahore, Pakistan
Received 23 November 2016, Accepted 07 April 2017
Abstract
Introduction

The present study was designed to investigate the association between rs8177374 polymorphism and malaria symptoms due to exposure of Plasmodium vivax and Plasmodium falciparum.

Materials and methods

A total of 454 samples were included in the study (228 malaria patients and 226 healthy individuals). Malaria patients, divided into P. vivax and P. falciparum groups on the basis of the causative species of Plasmodium, were categorized into mild and severe on the basis of clinical outcomes according to WHO criteria. Healthy individuals were used as controls. Allele specific PCR based strategy was used for the identification of rs8177374 SNP.

Results

MyD88-adaptor-like gene polymorphism was associated with susceptibility to malaria (p<0.001). C allele frequency (0.74) was higher in the population compared to T allele frequency (0.26). CT genotype increased the susceptibility of malaria (OR: 2.661; 95% CI: 1.722–4.113) and was positively associated with mild malaria (OR: 5.609; 95% CI: 3.479–9.044, p=0.00). On the other hand, CC genotype was associated with severe malaria (OR: 3.116; 95% CI: 1.560–6.224, p=0.00). P. vivax infection rate was higher in CT genotype carriers compared to other genotypes (OR: 3.616; 95% CI: 2.219–5.894, p<0.001).

Conclusion

MyD88-adaptor-like/TIR domain containing adaptor protein polymorphism for single nucleotide polymorphism rs8177374 is related with the susceptibility of malaria.

Keywords
Malaria, Plasmodium, rs8177374, MAL, Pakistani population
Braz J Infect Dis 2017;21:418-23 - Vol. 21 Num.4 DOI: 10.1016/j.bjid.2017.04.002