The Brazilian Journal of Infectious Diseases The Brazilian Journal of Infectious Diseases
Braz J Infect Dis 2017;21:155-61 - Vol. 21 Num.2 DOI: 10.1016/j.bjid.2016.10.011
Original article
Imbalance of Th17 and Treg in peripheral blood mononuclear cells of active tuberculosis patients
Jie Luoa, Mingxu Zhanga, Baosong Yana, Kejun Zhang, Ming Chen, , Shaoli Deng,
The Third Military Medical University, Institute of Surgery Research, Department of Clinical Laboratory Medicine, Chongqing, China
Received 13 August 2016, Accepted 13 October 2016
Abstract
Objective

Maintaining a right balance between Th17 and Treg might be critical to the immunopathogenesis of active tuberculosis (TB). This study aimed to assess whether the Th17/Treg balance is altered in active TB patients.

Methods

250 study subjects (90 active TB patients, 80 latent TB subjects, and 80 healthy controls) were recruited for the study. The expression of Th17 and Treg in peripheral blood mononuclear cells (PBMCs) in the 250 subjects was investigated by flow cytometry. Plasma levels of cytokines IL-17 and IL-10, which are related to Th17 and Treg, respectively, were determined by ELISA.

Results

The percentages of Th17 and Treg in PBMCs from active TB patients were significantly higher than those from latent TB or control groups (Th17: 4.31±1.35% vs. 1.58±0.71% or 1.15±0.49%, p<0.05; Treg: 11.44±2.69% vs. 7.54±1.56% or 4.10±0.99%, p<0.05). The expression of IL-17 and IL-10 was significantly increased in active TB patients in comparison to that in latent TB or control groups (IL-17: 16.85±9.68 vs. 7.23±5.19 or 8.21±5.51pg/mL, p<0.05; IL-10: 28.70±11.27 vs. 20.25±8.57 or 13.94±9.00pg/mL, p<0.05).

Conclusions

Our study demonstrated an altered balance of Treg/Th17 in active TB patients, with higher percentages of Th17 and Treg in PBMCs. Further research on this imbalance may offer a new direction for TB treatment.

Keywords
Active tuberculosis, Th17, Treg, IL-17, IL-10
Braz J Infect Dis 2017;21:155-61 - Vol. 21 Num.2 DOI: 10.1016/j.bjid.2016.10.011