The Brazilian Journal of Infectious Diseases The Brazilian Journal of Infectious Diseases
Braz J Infect Dis 2017;21:464-7 - Vol. 21 Num.4 DOI: 10.1016/j.bjid.2017.04.001
Brief communication
Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV and V isolated from healthy children attending public daycares in northeastern Brazil
Suzi P. de Carvalhoa,b, Jéssica B. de Almeidaa,b, Yasmin M.F.S. Andradeb, Lucas S.C. da Silvab, Arianne C. de Oliveirab, Flávia S. Nascimentob, Guilherme B. Camposc, Márcio V. Oliveirab, Jorge Timenetskyc, Lucas M. Marquesa,b,c,,
a Universidade Estadual de Santa Cruz, Departamento de Microbiologia, Ilhéus, BA, Brazil
b Universidade Federal da Bahia, Instituto Multidisciplinar em Saúde, Núcleo de Tecnologia em Saúde, Vitória da Conquista, BA, Brazil
c Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil
Received 24 August 2016, Accepted 17 April 2017
Abstract

Nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA) have increasingly been reported in healthy communities. This study aimed to assess the rate of S. aureus in general and MRSA in particular from nasal secretion of children in daycare centers in Vitória da Conquista, Brazil. The isolates were identified based on morphology, biochemical tests and by PCR. Detection of virulence genes, biofilm production, and susceptibility test by disk diffusion agar were performed. MRSA isolates were characterized by spa, SCCmec, and multilocus sequence typing (MLST). S. aureus were recovered from 70 (47.3%) of 148 children. Among the 11 MRSA strains (15.7%), two SCCmec types (IV and V) were detected. MLST identified four STs related to three clonal complexes (CC): 5, 45, and 398. Four spa types were found circulating in this setting. Resistance of S. aureus isolates to ampicillin, erythromycin, ciprofloxacin, clindamycin, and tetracycline was 80%, 32.8%, 7.1%, 7.1% and 4.3%, respectively. One isolate presented intermediate resistance to vancomycin detected by Etest methodology. All strains were biofilm producers. The virulence genes seb, sec, spa, and pvl were detected in some isolates. This study revealed a high rate of children carrying MRSA among healthy attendees in daycare centers in Vitória da Conquista, Brazil.

Keywords
CA-MRSA, Antibiotic resistance, SCCmec, MLST
Braz J Infect Dis 2017;21:464-7 - Vol. 21 Num.4 DOI: 10.1016/j.bjid.2017.04.001