Journal Information
Vol. 20. Issue 5.
Pages 413-524 (September - October 2016)
Share
Share
Download PDF
More article options
Vol. 20. Issue 5.
Pages 413-524 (September - October 2016)
Letter to the editor
DOI: 10.1016/j.bjid.2016.07.008
Open Access
Nephrin gene expression in chronic kidney disease of dogs with Leishmania (Leishmania) infantum chagasi
Visits
1503
Mariana de Medeiros Torres, Cristiane Silva Chitarra, Luciano Nakazato, Arleana Bom Parto Ferreira de Almeida, Valéria Régia Franco Sousa
Corresponding author
valeriaregia27@gmail.com

Corresponding author.
Universidade Federal de Mato Grosso (UFMT), Faculdade de Medicina Veterinária, Departmento de Medicina Veterinária Clínica, Cuiabá, MT, Brazil
This item has received
1503
Visits

Under a Creative Commons license
Article information
Full Text
Bibliography
Download PDF
Statistics
Full Text
Dear Editor,

Visceral leishmaniasis caused by Leishmania (Leishmania) infantum chagasi is the most severe form of the disease. In Brazil, this disease is transmitted through the bite of sand flies of the genus Lutzomyia.1 The clinical signs of the disease in canines, which are similar to those in humans, depend on the individual's humoral and cellular response, and kidney disease is the main cause of death.2 In visceral leishmaniasis nephropathies caused by glomerular diseases are a common cause of chronic renal failure.2–4 Glomerular podocyte injury causes proteinuria and results in tubular and interstitial injury.5 Thus, the aim of the study was to quantify nephrin gene expression in dogs suffering from chronic kidney disease (CKD) associated with visceral leishmaniasis.

Sixty-nine dogs with CKD were diagnosed based on their history, the presence of azotemia and isosthenuria, and were grouped according to classification proposed by the International Renal Interest Society (IRIS). Urine, blood and bone marrow samples were collected by jugular venipuncture, cystocentesis and/or urethral catheter, and sternal puncture, respectively.

This study was approved by and conducted according to the guidelines of the Ethics Committee on Animal Use of the Federal University of Mato Grosso (Protocol # 23108.043331/12-9). The DNA extracted from blood and bone marrow was subjected to PCR detection of L. (L.) infantum chagasi. Cell pellets were obtained by centrifuging 10mL of urine for RNA extraction, followed by RT-qPCR to quantify nephrin gene expression. Supernatant from centrifuged urine was used for biochemical determination of proteinuria.

Nephrin was quantified in the urinary sediment of 47 dogs and L. (L.) infantum chagasi was detected in the bone marrow and/or blood of 11 dogs. The relative quantitation of nephrin was lower in dogs in advanced stages of renal disease than in the initial stages, and those positive for L. L. infantum chagasi showed no correlation with the presence of the parasite (r=−0.35).

The quantitation of nephrin expression according to the stage of CKD and its association with L. L. infantum chagasi have not been reported previously. Kidney disease is an important condition in the clinical assessment of small animals, in view of the high prevalence and severity of its subsequent clinical manifestations. Moreover, owing to the zoonotic potential of visceral leishmaniasis associated with the presence of asymptomaticity in dogs, the early detection of kidney disease enables the evolution of the disease to be monitored.

Visceral leishmaniasis impairs the immune system and interferes with the inflammatory response, resulting in several changes that culminate in death from renal failure. Knowing that canine cases precede the occurrence of the disease in humans, it is important to identify the changes that may occur in both species. Therefore, dogs also serve as a study model of this disease, assisting in the establishment of a more accurate prognosis for humans, and is a control measure that requires fast and accurate diagnostic tools. Hence, the quantitation of nephrin gene expression in urinary sediment is a practical and non-invasive tool for monitoring the development of kidney disease.

Conflicts of interest

The authors declare no conflicts of interest.

References
[1]
M.S. Latrofa, F. Dantas-Torres, D. Caprariis, et al.
Vertical transmission of Anaplasma platys and Leishmania infantum in dogs during the first half of gestation.
Parasit Vectors, 9 (2016), pp. 269
[2]
A.F. Koutinas, C.K. Koutinas.
Pathologic mechanisms underlying the clinical findings in canine leishmaniosis due to Leishmania infantum/chagasi.
Vet Path, 51 (2014), pp. 527-538
[3]
J. Patrakka, K. Tryggvason.
Molecular make-up of the glomerular filtration barrier.
Biochem Biophys Res Commun, 396 (2010), pp. 164-169
[4]
R.S. Rigo, C.M.E. Carvalho, M.R. Honer, et al.
Renal histopathological findings in dogs with visceral leishmaniasis.
Rev Inst Med Trop, 55 (2013), pp. 113-116
[5]
R. Kobayashi, J. Kamiie, K. Yasuno, K. Ogihara, K. Shirota.
Expression of Nephrin, Podocin, a-Actinin-4 and a3-Integrin in canine renal glomeruli.
J Comp Path, 145 (2011), pp. 220-225
Copyright © 2016. Sociedade Brasileira de Infectologia
The Brazilian Journal of Infectious Diseases

Subscribe to our newsletter

Article options
Tools
Cookies policy
To improve our services and products, we use cookies (own or third parties authorized) to show advertising related to client preferences through the analyses of navigation customer behavior. Continuing navigation will be considered as acceptance of this use. You can change the settings or obtain more information by clicking here.