TY - JOUR T1 - Virus isolation and genotype identification of human respiratory syncytial virus in Guizhou Province, China JO - The Brazilian Journal of Infectious Diseases T2 - AU - Zhao,Ting AU - Ye,Zhixu AU - Wang,Binlin AU - Cui,Yuxia AU - Nie,Yingjie AU - Yang,Bin AU - Chen,Kun AU - Zhang,Hua AU - Hu,Fangfang AU - Yu,Fuxun SN - 14138670 M3 - 10.1016/j.bjid.2019.10.007 DO - 10.1016/j.bjid.2019.10.007 UR - https://bjid.org.br/en-virus-isolation-genotype-identification-human-articulo-S1413867019304738 AB - To investigate the genetic variation and molecular epidemiology characteristics of Human Respiratory Syncytial Virus (HRSV) in Guizhou Province, nasopharyngeal aspirates were collected from patients with acute respiratory infection (ARI) in Guizhou Provincial People's Hospital, from December 2017 to March 2018, and inoculated to Hep-2 cells to isolate HRSV. Cells that showed cytopathic effect (CPE) were then confirmed by indirect immunofluorescence assay and reverse transcription. The sequence of the PCR products was determined for HRSV isolates, and the genetic variation was analyzed. Out of 196 nasopharyngeal aspirate samples, HRSV were isolated in 39. The second hypervariable region at the 3′ terminal of glycoprotein gene (HVR2) sequence analysis showed that subgroup A was dominant. Seventy-nine percent of the isolates belonged to subgroup A, ON1 genotype, and 21 % belonged to subgroup B, BA9 genotype, which indicates that the dominant HRSV circulating in Guizhou Province was subgroup A, genotype ON1, co-circulating with a less prevalent subgroup B, genotype BA9. ER -