TY - JOUR T1 - Phylogenetic analysis of the emergence of main hepatitis C virus subtypes in São Paulo, Brazil JO - The Brazilian Journal of Infectious Diseases T2 - AU - Nishiya,Anna Shoko AU - de Almeida-Neto,César AU - Romano,Camila Malta AU - Alencar,Cecília Salete AU - Ferreira,Suzete Cleusa AU - Di-Lorenzo-Oliveira,Claudia AU - Levi,José Eduardo AU - Salles,Nanci Alves AU - Mendrone-Junior,Alfredo AU - Sabino,Ester Cerdeira SN - 14138670 M3 - 10.1016/j.bjid.2015.06.010 DO - 10.1016/j.bjid.2015.06.010 UR - https://bjid.org.br/en-phylogenetic-analysis-emergence-main-hepatitis-articulo-S1413867015001336 AB - BackgroundIt is recognized that hepatitis C virus subtypes (1a, 1b, 2a, 2b, 2c and 3a) originated in Africa and Asia and spread worldwide exponentially during the Second World War (1940) through the transfusion of contaminated blood products, invasive medical and dental procedures, and intravenous drug use. The entry of hepatitis C virus subtypes into different regions occurred at distinct times, presenting exponential growth rates of larger or smaller spread. Our study estimated the growth and spread of the most prevalent subtypes currently circulating in São Paulo. MethodsA total of 465 non-structural region 5B sequences of hepatitis C virus covering a 14-year time-span were used to reconstruct the population history and estimate the population dynamics and Time to Most Recent Common Ancestor of genotypes using the Bayesian Markov Chain Monte Carlo approach implemented in BEAST (Bayesian evolutionary analysis by sampling tree software/program). ResultsEvolutionary analysis demonstrated that the different hepatitis C virus subtypes had distinct growth patterns. The introduction of hepatitis C virus-1a and -3a were estimated to be circa 1979 and 1967, respectively, whereas hepatitis C virus-1b appears to have a more ancient entry, circa 1923. Hepatitis C virus-1b phylogenies suggest that different lineages circulate in São Paulo, and four well-supported groups (i.e., G1, G2, G3 and G4) were identified. Hepatitis C virus-1a presented the highest growth rate (r=0.4), but its spread became less marked after the 2000s. Hepatitis C virus-3a grew exponentially until the 1990s and had an intermediate growth rate (r=0.32). An evident exponential growth (r=0.26) was found for hepatitis C virus-1b between 1980 and the mid-1990s. ConclusionsAfter an initial period of exponential growth, the expansion of the three main subtypes began to decrease. Hepatitis C virus-1b presented inflated genetic diversity, and its transmission may have been sustained by different generations and transmission routes other than blood transfusion. Hepatitis C virus-1a and -3a showed no group stratification, most likely due to their recent entry. ER -