Detection of SPM and IMP metallo-β-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital

Camargo, Carlos Henrique; Bruder-Nascimento, Ariane; Mondelli, Alessandro Lia; Montelli, Augusto Cezar; Sadatsune, Terue

doi:10.1016/S1413-8670(11)70231-8

The Brazilian Journal of Infectious Diseases

ISSN: 1413-8670

The Brazilian Journal of Infectious Diseases is the official publication of the Brazilian Society of Infectious Diseases (SBI). It aims to publish relevant articles in the broadest sense on all aspects of microbiology, infectious diseases and immune response to infectious agents.
The BJID is a bimonthly publication and one of the most influential journals in its field in Brazil and Latin America with a high impact factor. Since its inception it has garnered a growing share of the publishing market.

Indexed in:

CABI Publishing; Chemical Abstracts (CAS); China Education Publications Import and Export Corporation (CEPIEC); EBSCO Database (Premium Research); Elsevier Science - Bibliographic Databases Division; Embase/Excerpta Medica; Gale Cengage Learning; Index Copernicus International; ISI (Web of Science) - Science Citation Index Expanded (SCISEARCH); Journal Citation Reports (Science Edition); Kessler-Hancok Information Service; Latin America and Caribean Literature of Health Science (LILACS); LATINDEX; Medline/ Index Medicus/Pubmed; Qualis International (CAPES): B3 (International Quality); Redalyc (Network of Scientific Journals of Latin America, Caribbean, Spain and Portugal) SCIELO; SCIRUS (Elsevier); SCOPUS Index; Sociedad Iberoamericana de Información Cientifica (SIIC); Subis Database (SUBIS Bulletin); Swets Blackwell; Teldan Database Production; The US Library of Congress Office; Ulrich¿s Periodicals Directory.

Phenotypic and genotypic SPM and IMP metallo-β-lactamases (MBL) detection and also the determination of minimal inhibitory concentrations (MIC) to imipenem, meropenem and ceftazidime were evaluated in 47 multidrug-resistant Pseudomonas aeruginosa isolates from clinical specimens. Polymerase chain reaction detected 14 positive samples to either blaSPM or blaIMP genes, while the best phenotypic assay (ceftazidime substrate and mercaptopropionic acid inhibitor) detected 13 of these samples. Imipenem, meropenem and ceftazidime MICs were higher for MBL positive compared to MBL negative isolates. We describe here the SPM and IMP MBL findings in clinical specimens of P. aeruginosa from the University Hospital of Botucatu Medical School, São Paulo, Brazil, that reinforce local studies showing the high spreading of blaSPM and blaIMP genes among Brazilian clinical isolates.

Keywords:

Pseudomonas aeruginosa

drug resistance, bacterial

carbapenems

metalloproteins

polymerase chain reaction

Full text is only aviable in PDF

References

[1.]

D. Livermore.

Beta-Lactamases in laboratory and clinical resistance.

Clin Microbiol Rev, 8 (1995), pp. 557-584

Medline

[2.]

K. Bush, G. Jacoby.

Updated functional classification of beta-lactamases.

Antimicrob Agents Chemother, 54 (2010), pp. 969-976

http://dx.doi.org/10.1128/AAC.01009-09 | Medline

[3.]

A. Zavascki, A. Barth, A. Gonçalves, et al.

The influence of metallo-beta-lactamase production on mortality in nosocomial Pseudomonas aeruginosa infections.

J Antimicrob Chemother, 58 (2006), pp. 387-392

http://dx.doi.org/10.1093/jac/dkl239 | Medline

[4.]

A. Mastoraki, E. Douka, I. Kriaras, et al.

Pseudomonas aeruginosa susceptible only to colistin in intensive care unit patients.

Surg Infect (Larchmt), 9 (2008), pp. 153-160

[5.]

Y. Lee, B. Ahn, J. Jin, et al.

Molecular characterization of Pseudomonas aeruginosa isolates resistant to all antimicrobial agents, but susceptible to colistin, in Daegu, Korea.

J Microbiol, 45 (2007), pp. 358-363

Medline

[6.]

T. Walsh, M. Toleman, L. Poirel, et al.

Metallo-beta-lactamases: the quiet before the storm?.

Clin Microbiol Rev, 18 (2005), pp. 306-325

http://dx.doi.org/10.1128/CMR.18.2.306-325.2005 | Medline

[7.]

R. Picão, S. Andrade, A. Nicoletti, et al.

Metallo-beta-lactamase detection: comparative evaluation of double-disk synergy versus combined disk tests for IMP-, GIM-, SIM-, SPM-, or VIM-producing isolates.

J Clin Microbiol, 46 (2008), pp. 2028-2037

http://dx.doi.org/10.1128/JCM.00818-07 | Medline

[8.]

J. Pitout, D. Gregson, L. Poirel, et al.

Detection of Pseudomonas aeruginosa producing metallo-beta-lactamases in a large centralized laboratory.

J Clin Microbiol, 43 (2005), pp. 3129-3135

http://dx.doi.org/10.1128/JCM.43.7.3129-3135.2005 | Medline

[9.]

H. Sader, A. Reis, S. Silbert, A. Gales.

IMPs, VIMs and SPMs: the diversity of metallo-beta-lactamases produced by carbapenemresistant Pseudomonas aeruginosa in a Brazilian hospital.

Clin Microbiol Infect, 11 (2005), pp. 73-76

http://dx.doi.org/10.1111/j.1469-0691.2004.01031.x | Medline

[10.]

R. Picão, L. Poirel, A. Gales, et al.

Diversity of beta-lactamases produced by ceftazidime-resistant Pseudomonas aeruginosa isolates causing bloodstream infections in Brazil.

Antimicrob Agents Chemother, 53 (2009), pp. 3908-3913

http://dx.doi.org/10.1128/AAC.00453-09 | Medline

[11.]

Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 20th informational supplement. M100-S20. Wayne, PA: Clinical and Laboratory Standards Institute; 2010.

[12.]

Y. Doi, A. Ghilardi, J. Adams, et al.

High prevalence of metallobeta-lactamase and 16S rRNA methylase coproduction among imipenem-resistant Pseudomonas aeruginosa isolates in Brazil.

Antimicrob Agents Chemother, 51 (2007), pp. 3388-3390

http://dx.doi.org/10.1128/AAC.00443-07 | Medline

[13.]

R. Bonomo, D. Szabo.

Mechanisms of multidrug resistance in Acinetobacter species and Pseudomonas aeruginosa.

Clin Infect Dis, 43 (2006), pp. S49-S56

http://dx.doi.org/10.1086/504477 | Medline

[14.]

V. Viana Vieira, E. Lourenço da Fonseca, A. Paulo Vincente.

Metallo-beta-lactamases produced by carbapenem-resistant Pseudomonas aeruginosa in Brazil.

Clin Microbiol Infect, 11 (2005), pp. 937

http://dx.doi.org/10.1111/j.1469-0691.2005.01249.x | Medline

[15.]

E. Fonseca, F. Freitas, A. Vicente.

The colistin-only-sensitive (COS) Brazilian Pseudomonas aeruginosa clone SP (ST 277) is spread worldwide.

Antimicrob Agents Chemother, 54 (2010), pp. 2743

http://dx.doi.org/10.1128/AAC.00012-10 | Medline

[16.]

A. Gales, L. Menezes, S. Silbert, et al.

Dissemination in distinct Brazilian regions of an epidemic carbapenemresistant Pseudomonas aeruginosa producing SPM metallo-beta-lactamase.

J Antimicrob Chemother, 52 (2003), pp. 699-702

http://dx.doi.org/10.1093/jac/dkg416 | Medline

[17.]

A. Martins, A. Zavascki, P. Gaspareto, et al.

Dissemination of Pseudomonas aeruginosa producing SPM-1-like and IMP-1-like metallo-beta-lactamases in hospitals from southern Brazil.

Infection, 35 (2007), pp. 457-460

http://dx.doi.org/10.1007/s15010-007-6289-3 | Medline

[18.]

L. Poirel, M. Magalhaes, M. Lopes, et al.

Molecular analysis of metallo-beta-lactamase gene bla (SPM-1)-surrounding sequences from disseminated Pseudomonas aeruginosa isolates in Recife. Brazil.

Antimicrob Agents Chemother, 48 (2004), pp. 1406-1409

Medline

[19.]

T. Gräf, D. Fuentefria, G. Corção.

Occurrence of multiresistant strains of Pseudomonas aeruginosa producing metallo-beta-lactamase blaSPM-1 in clinical samples.

Rev Soc Bras Med Trop, 41 (2008), pp. 306-308

Medline

[20.]

A. Salabi, M. Toleman, J. Weeks, et al.

First report of the metallo-beta-lactamase SPM-1 in Europe.

Antimicrob Agents Chemother, 54 (2010), pp. 582

http://dx.doi.org/10.1128/AAC.00719-09 | Medline

Indexed in:

Follow us:

Subscribe to our newsletter